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Chemical Sciences
Introduction
Objective
List of experiments
Target Audience
Course Alignment
Feedback
Introduction
Objective
List of experiments
Target Audience
Course Alignment
Feedback
Molecular Fluorescence Spectroscopy
Introduction to the fluorescence spectroscopy principle
Familiarization with the fluorescence Instrumentation
Familiarization with Excitation and Emission Spectra, Mirror Image Relation and Stokes Shift
Demonstration of Solvent Effects on Fluorescence Spectra of a Fluorophore
Demonstration of pH Effect on Fluorescence Excitation and Emission Spectra of a Fluorophore
Effects of Fluorophore Concentration on Fluorescence Spectra: Inner Filter Effects
Relationship between the Concentration of a Fluorophore and its Fluorescence Intensity: Determination of Unknown Concentration of an Analyte
Determination of Fluorescence Quantum Yield of a Fluorophore
Fluorescence Quenching and Stern-Volmer Plot: Estimation of the Quenching Constant from Stern-Volmer Plot
Effects of Excimer and Exciplex Formations on Fluorescence Emission
